Abstract
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biosensing Techniques / methods*
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Brain / cytology
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Brain / embryology
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Brain / metabolism*
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Calcium / metabolism*
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Calcium Signaling*
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Dendrites / metabolism
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Female
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Mice, Transgenic
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Neurons / metabolism*
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Spectrometry, Fluorescence
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Troponin C / genetics
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Troponin C / metabolism*
Substances
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Cyan Fluorescent Protein
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Troponin C
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Green Fluorescent Proteins
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Calcium