Abstract
To study the interaction between an invading virus and its host, we investigated differential gene expression in mandarin fish Siniperca chuatsi experimentally infected with infectious spleen and kidney necrosis virus (ISKNV). Subtractive cDNA libraries were constructed by suppression subtractive hybridization (SSH) from spleens of mock- and ISKNV-infected fish. Both forward- and reverse-subtracted libraries were generated. In the forward library, genes of the ubiquitin-proteasome proteolytic pathway, defense-related genes, a cytoskeletal protein gene, an apoptosis-related gene encoding inhibitor of apoptosis protein and JFC/EBPb cDNA for CAAT/Enhancer binding protein beta were up-regulated after infection. In the reverse library, genes that encoded CD59/neurotoxin/Ly-6-like protein, carboxypeptidase A2, and goose-type lysozyme were down-regulated. Some of these genes were analyzed by reverse transcription-polymerase chain reaction to confirm their differential expression as a result of virus infection. The results of this study may contribute to our understanding of fish innate immune response to ISKNV.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CCAAT-Enhancer-Binding Protein-beta / genetics
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CCAAT-Enhancer-Binding Protein-beta / metabolism
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Consensus Sequence
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Cytokines / genetics
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Cytokines / metabolism
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DNA Primers / chemistry
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DNA Virus Infections / immunology
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DNA Virus Infections / veterinary*
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DNA Virus Infections / virology
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Fish Diseases / immunology*
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Fish Diseases / virology
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Gene Expression / immunology*
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Gene Expression Profiling
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Gene Library
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Inhibitor of Apoptosis Proteins / genetics
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Inhibitor of Apoptosis Proteins / metabolism
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Iridoviridae / immunology*
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Iridoviridae / pathogenicity
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Nucleic Acid Hybridization
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Perciformes / genetics*
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Perciformes / virology
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RNA, Messenger / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Sequence Analysis, DNA
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Spleen / immunology*
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Spleen / physiopathology
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Talin / genetics
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Talin / metabolism
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Ubiquitin / genetics
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Ubiquitin / metabolism
Substances
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CCAAT-Enhancer-Binding Protein-beta
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Cytokines
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DNA Primers
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Inhibitor of Apoptosis Proteins
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RNA, Messenger
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Ribosomal Proteins
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Talin
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Ubiquitin