New rapid staining methods of Cryptosporidium oocysts in stools

J Protozool. 1991 Nov-Dec;38(6):212S-214S.

Abstract

Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / parasitology
  • Animals
  • Cryptosporidium / isolation & purification*
  • Evaluation Studies as Topic
  • Feces / parasitology*
  • HIV Seropositivity / parasitology
  • Humans
  • Male
  • Merbromin*
  • Sensitivity and Specificity
  • Staining and Labeling / methods*

Substances

  • Merbromin