Enzymatic fragment substitution as a tool in protein design

Biomed Biochim Acta. 1991;50(10-11):S175-80.

Abstract

An easy and rapid enzymatic method is described which allows replacement of P'-residues in bovine pancreatic trypsin inhibitor. Insertion of Xaa-Arg or Xaa-Lys into a BPTI fragment lacking P1' = Ala16 and P2' = Arg17 was carried out in a "one pot" reaction catalysed by trypsin in the presence of 80% 1,4 butanediol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aprotinin / chemical synthesis
  • Aprotinin / chemistry*
  • Binding Sites
  • Cattle
  • Drug Design*
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Trypsin / chemistry

Substances

  • Peptide Fragments
  • Aprotinin
  • Trypsin