Abstract
We report the identification of a cDNA that encodes a putative protein of 94 amino acids and expected molecular weight of 10.7 kDa, the C-terminal half of which is identical to that of PEP19, a small, brain-specific protein involved in Ca++/calmodulin signaling. The novel rat-specific protein, tentatively named long PEP19 isoform (LPI), is the product of alternative splicing of the rat PCP4 gene encoding PEP19. We found that antibodies raised against the first 13 N-terminal amino acids of LPI, not present in PEP19, recognize a protein enriched in the developing rat brain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies / immunology
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Base Sequence
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Brain / metabolism
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Calmodulin-Binding Proteins
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Cell Extracts
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Exons / genetics
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Gene Expression Profiling
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Gene Expression Regulation, Developmental
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Humans
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Introns / genetics
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / immunology
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Nerve Tissue Proteins / metabolism
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Peptides / immunology
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / immunology
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Protein Isoforms / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / immunology
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RNA-Binding Proteins / metabolism
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Rats
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Rats, Sprague-Dawley
Substances
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Antibodies
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Calmodulin-Binding Proteins
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Cell Extracts
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DNA, Complementary
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Nerve Tissue Proteins
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Pcp4 protein, rat
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Peptides
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Protein Isoforms
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RNA, Messenger
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RNA-Binding Proteins