Abstract
The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (6b) against replication of vesicular stomatitis virus (VSV) in Vero cells was investigated. Time-related experiments showed that 6b mainly affects a late event of the virus growth cycle. Virus adsorption, internalisation and early RNA synthesis are not the target of the inhibitory action. Results obtained indicate that the antiviral compound adversely affects virus protein synthesis and viral mature particle formation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / chemical synthesis
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Antiviral Agents / chemistry
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Antiviral Agents / pharmacology*
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Base Sequence
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Chlorocebus aethiops
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DNA, Viral / genetics
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Molecular Structure
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RNA, Viral / biosynthesis
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RNA, Viral / genetics
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Steroids / chemical synthesis
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Steroids / chemistry
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Steroids / pharmacology*
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Vero Cells
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Vesicular stomatitis Indiana virus / drug effects*
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Vesicular stomatitis Indiana virus / genetics
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Vesicular stomatitis Indiana virus / physiology
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Virus Replication / drug effects*
Substances
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Antiviral Agents
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DNA, Viral
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RNA, Viral
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Steroids