Combination treatment with donor interleukin-12p35 silenced dendritic cells and cyclosporine induces long-term survival of intestinal allografts in rats

H Xu; T Chen; H Q Wang; Y B Hua; W W Wu

doi:10.1016/j.transproceed.2006.10.229

Combination treatment with donor interleukin-12p35 silenced dendritic cells and cyclosporine induces long-term survival of intestinal allografts in rats

Transplant Proc. 2007 Jan-Feb;39(1):286-8. doi: 10.1016/j.transproceed.2006.10.229.

Authors

H Xu¹, T Chen, H Q Wang, Y B Hua, W W Wu

Affiliation

¹ Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

PMID: 17275524
DOI: 10.1016/j.transproceed.2006.10.229

Abstract

Immune tolerance promises to enhance allograft survival while reducing risks inherent to immunosuppressants. In this study, we evaluated the effect of interleukin (IL)-12p35 silenced dendritic cells (DC) combined with cyclosporine (CsA) on induction of immune tolerance in intestinal transplantation. The results showed that combination treatment with donor IL-12p35 silenced DC and a low dose of CsA (8 mg/kg) prolonged rat intestinal allograft survival. However, a higher dose of CsA (20 mg/kg) did not further prolong allograft survival.

MeSH terms

Animals
Combined Modality Therapy
Cyclosporine / therapeutic use*
Dendritic Cells / immunology*
Dendritic Cells / transplantation*
Gene Silencing*
Graft Survival / immunology
Interleukin-12 Subunit p35 / genetics*
Intestine, Small / transplantation*
Intestines / transplantation*
Male
Models, Animal
Rats
Rats, Inbred WF
Rats, Sprague-Dawley
Survival
Transplantation, Homologous / immunology*

Substances

Interleukin-12 Subunit p35
Cyclosporine