Abstract
Group B streptococcus (GBS) expresses a hemolysin/cytolysin that plays an important role in pathogenesis. Using the Himar1 transposon mutagenesis system, a hypohemolytic mutant carrying an interrupted cylJ gene was characterized. cylJ, encoding a putative glycosyltransferase, and cylK, whose product is unknown, are both required for the full hemolytic/cytolytic activity, pigment formation, and virulence of GBS.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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DNA Transposable Elements
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Economics
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Gene Deletion
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Gene Expression Regulation, Bacterial
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Genes
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Genes, Bacterial*
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Glucosyltransferases / genetics*
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Hemolysin Proteins / biosynthesis*
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Mutagenesis, Insertional
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Pigments, Biological / biosynthesis
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Rats
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Streptococcal Infections / microbiology
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Streptococcus
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Streptococcus agalactiae / genetics
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Streptococcus agalactiae / metabolism
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Streptococcus agalactiae / pathogenicity*
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Virulence / genetics
Substances
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Bacterial Proteins
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DNA Transposable Elements
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Hemolysin Proteins
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Pigments, Biological
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Glucosyltransferases