Interleukin-8 and CXCR1 receptor functional polymorphisms and susceptibility to acute pyelonephritis

J Urol. 2007 Mar;177(3):1102-6. doi: 10.1016/j.juro.2006.10.037.

Abstract

Purpose: We performed a case-control study in children diagnosed by the first episode of upper urinary tract infection with or without vesicoureteral reflux to evaluate the association of functional polymorphism of interleukin-8 (-251A>T and +2767A>G), and its receptor CXCR1 (+2607G>C).

Materials and methods: Genomic DNA was obtained from 265 children with a clinical and laboratory diagnosis of urinary tract infection who were recruited in northeast Italy. The children were subdivided as 173 who were dimercapto-succinic acid scan positive with positive static renal scintigraphy in acute conditions, consistent with the diagnosis of acute pyelonephritis, and 92 who were dimercapto-succinic acid scan negative. Genetic analysis for the same polymorphisms was also extended to a control population of 106 umbilical cord DNA samples.

Results: Statistical analysis of genotype data showed that 1) the tested populations were in Hardy-Weinberg equilibrium, 2) there were significant differences between the dimercapto-succinic acid scan positive and negative groups (p=0.049), and the dimercapto-succinic acid scan positive group vs controls (p=0.032) in terms of interleukin-8 -251A>T polymorphism frequency, 3) there was also a significant difference in the distribution of IL-8 -251A>T and +2767A>G polymorphisms between dimercapto-succinic acid scan positive and negative children in the subgroup without vesicoureteral reflux (p=0.03 and 0.02, respectively) and 4) no significant differences were found in the frequency of the distribution of CXCR1 +2607G>C polymorphism in all groups.

Conclusions: These data suggest that the gene for the proinflammatory chemokine interleukin-8 is involved in susceptibility to acute pyelonephritis during upper urinary tract infection in children with or without vesicoureteral reflux.

MeSH terms

  • Acute Disease
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Infant
  • Infant, Newborn
  • Interleukin-8 / genetics*
  • Male
  • Polymorphism, Genetic / genetics*
  • Pyelonephritis / genetics*
  • Receptors, Interleukin-8A / genetics*
  • Urinary Tract Infections / complications
  • Urinary Tract Infections / genetics*
  • Vesico-Ureteral Reflux / complications
  • Vesico-Ureteral Reflux / genetics

Substances

  • Interleukin-8
  • Receptors, Interleukin-8A