Abstract
Deficient motility is one of the characteristic hallmarks observed in Pseudomonas aeruginosa strains that chronically colonize the lungs of cystic fibrosis (CF) patients. Pseudomonas aeruginosa TB is a nonpiliated CF isolate known to be defective in twitching motility. Complementation confirmed a direct link of this phenotype to an intragenic out-of-frame deletion in pilQ (PA5040). Sequence alignment of pilQ derived from TB vs. PAO1 suggests that close direct repeats framing the deletion site may have triggered this mutation. This type of mutation could play a role in the emergence of pathoadaptive mutations of P. aeruginosa in the CF lung habitat.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Bacterial Proteins / physiology
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Blotting, Southern
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Cystic Fibrosis / microbiology*
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Electrophoresis, Polyacrylamide Gel
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Fimbriae Proteins / genetics*
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Fimbriae Proteins / metabolism
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Fimbriae Proteins / physiology
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Gene Deletion*
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Gene Expression Regulation, Bacterial
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Humans
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Immunoblotting
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Lung / microbiology*
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Mutation
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Polymerase Chain Reaction
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / growth & development
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Pseudomonas aeruginosa / metabolism
Substances
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Bacterial Proteins
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pilQ protein, bacteria
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Fimbriae Proteins