Abstract
A simple, sensitive and specific liquid chromatography/tandem mass spectrometry method for the quantitation of aildenafil, a new phosphodiesterase V inhibitor, in human plasma is presented. The analyte and internal standard, sildenafil, were extracted by a one-step liquid-liquid extraction in alkaline conditions and separated on a C(18) column using ammonia:10mM ammonium acetate buffer:methanol (0.1:15:85, v/v/v) as the mobile phase. The detection by an API 4000 triple quadrupole mass spectrometer in multiple-reaction monitoring mode was completed within 2.5 min. The calibration curve exhibited a linear dynamic range of 0.05-100 ng/ml with a 10 pg/ml limit of detection. The intra- and inter-day precisions measured as relative standard deviation were within 8.04% and 5.72%, respectively. This method has been used in a pharmacokinetic study of aildenafil in healthy male volunteers each given an oral administration of one of the three dosages.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetates / chemistry
-
Administration, Oral
-
Ammonia / chemistry
-
Area Under Curve
-
Buffers
-
Calibration
-
Chromatography, Liquid / instrumentation
-
Chromatography, Liquid / methods*
-
Dose-Response Relationship, Drug
-
Fasting
-
Half-Life
-
Humans
-
Hydrogen-Ion Concentration
-
Male
-
Metabolic Clearance Rate
-
Methanol / chemistry
-
Molecular Structure
-
Phosphodiesterase Inhibitors / administration & dosage
-
Phosphodiesterase Inhibitors / blood*
-
Phosphodiesterase Inhibitors / chemistry
-
Phosphodiesterase Inhibitors / classification
-
Phosphodiesterase Inhibitors / isolation & purification
-
Phosphodiesterase Inhibitors / pharmacokinetics*
-
Piperazines / administration & dosage
-
Piperazines / blood*
-
Piperazines / chemistry
-
Piperazines / pharmacokinetics*
-
Purines
-
Reference Standards
-
Reproducibility of Results
-
Sensitivity and Specificity
-
Sildenafil Citrate
-
Sulfones / administration & dosage
-
Sulfones / blood*
-
Sulfones / chemistry
-
Sulfones / pharmacokinetics*
-
Tablets
-
Tandem Mass Spectrometry / methods*
-
Time Factors
Substances
-
Acetates
-
Buffers
-
Phosphodiesterase Inhibitors
-
Piperazines
-
Purines
-
Sulfones
-
Tablets
-
Ammonia
-
aildenafil
-
Sildenafil Citrate
-
ammonium acetate
-
Methanol