Activity of the notch-signalling pathway in circulating human chronic lymphocytic leukaemia cells

Scand J Immunol. 2007 Mar;65(3):271-5. doi: 10.1111/j.1365-3083.2006.01897.x.

Abstract

Dysregulation of the Notch-pathway has been implicated in the pathogenesis of chronic lymphocytic leukaemia (B-CLL). We characterized the mRNA expression of Notch pathway elements in circulating normal B- and B-CLL cells, and compared expression profiles with clinical and prognostic data. Similar expression profiles were found in normal B-cells and B-CLL cells, however, most B-CLL samples showed lower Hairy/Enhancer of Split-1 expression than normal B-cells, which suggests that the pathway is not over-activated in B-CLL. The expression of Notch-pathway genes did not correlate with other prognostic factors of B-CLL. The importance of Notch-signalling in CLL cells in lymphatic tissue microenvironments remains to be determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase 1 / metabolism
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism*
  • Blotting, Western
  • Flow Cytometry
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Immunoglobulin Heavy Chains
  • Leukemia, Lymphocytic, Chronic, B-Cell / metabolism*
  • Prognosis
  • RNA, Messenger / analysis
  • Receptors, Notch / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / immunology*
  • Somatic Hypermutation, Immunoglobulin

Substances

  • Immunoglobulin Heavy Chains
  • RNA, Messenger
  • Receptors, Notch
  • ADP-ribosyl Cyclase 1