A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1

Kazunori Kume; Tetsuya Goshima; Kohji Miyahara; Takashi Toda; Dai Hirata

doi:10.1271/bbb.60574

A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1

Biosci Biotechnol Biochem. 2007 Feb;71(2):615-7. doi: 10.1271/bbb.60574.

Authors

Kazunori Kume¹, Tetsuya Goshima, Kohji Miyahara, Takashi Toda, Dai Hirata

Affiliation

¹ Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.

PMID: 17317928
DOI: 10.1271/bbb.60574

Abstract

In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / metabolism*
Cell Cycle Proteins / analysis*
Electrophoresis, Polyacrylamide Gel
Green Fluorescent Proteins / genetics
Myelin Basic Protein / genetics
Protein Kinases / metabolism*
Protein Serine-Threonine Kinases
Schizosaccharomyces / enzymology*
Schizosaccharomyces pombe Proteins / analysis*
Schizosaccharomyces pombe Proteins / metabolism*
Ultracentrifugation

Substances

Carrier Proteins
Cell Cycle Proteins
Myelin Basic Protein
Pmo25 protein, S pombe
Schizosaccharomyces pombe Proteins
Green Fluorescent Proteins
Protein Kinases
Sid1 kinase
Nak1 protein, S pombe
Protein Serine-Threonine Kinases