Abstract
Objective:
To study IgG rheumatoid factor (RF) from rheumatoid synovium.
Methods:
We fused the K6H6/B5 human-mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF-secreting hybridomas.
Results:
The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium.
Conclusion:
IgG-RF-secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Arthritis, Rheumatoid / immunology*
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Arthritis, Rheumatoid / metabolism
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Cells, Cultured
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Enzyme-Linked Immunosorbent Assay
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Humans
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Hybridomas / immunology
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Hybridomas / pathology
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Immunoblotting
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Immunoglobulin G / analysis*
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Immunoglobulin G / immunology
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Immunoglobulin G / metabolism
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Mice
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Rheumatoid Factor / analysis*
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Rheumatoid Factor / immunology
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Rheumatoid Factor / metabolism
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Synovial Membrane / chemistry*
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Synovial Membrane / immunology
Substances
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Immunoglobulin G
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Rheumatoid Factor