Abstract
OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81alpha and OsMUS81beta. OsMus81alpha contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81beta isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and gamma-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing / genetics*
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Alternative Splicing / radiation effects
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Amino Acid Sequence
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Base Sequence
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DNA Damage* / radiation effects
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DNA, Plant
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Endonucleases / chemistry
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Endonucleases / genetics*
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Gene Expression Regulation, Plant* / radiation effects
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Oryza / genetics*
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Oryza / radiation effects
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics*
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Sequence Homology
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Two-Hybrid System Techniques
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Up-Regulation / genetics*
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Up-Regulation / radiation effects
Substances
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DNA, Plant
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DNA-Binding Proteins
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Plant Proteins
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Saccharomyces cerevisiae Proteins
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Endonucleases
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MUS81 protein, S cerevisiae