Cordycepin (3'-deoxyadenosine) inhibits human platelet aggregation induced by U46619, a TXA2 analogue

Hyun Jeong Cho; Jae Youl Cho; Man Hee Rhee; Chang Ryul Lim; Hwa Jin Park

doi:10.1211/jpp.58.12.0016

Cordycepin (3'-deoxyadenosine) inhibits human platelet aggregation induced by U46619, a TXA2 analogue

J Pharm Pharmacol. 2006 Dec;58(12):1677-82. doi: 10.1211/jpp.58.12.0016.

Authors

Hyun Jeong Cho¹, Jae Youl Cho, Man Hee Rhee, Chang Ryul Lim, Hwa Jin Park

Affiliation

¹ Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Regional Research Center, Inje University, Gimhae, Gyungnam 621-749, Korea.

PMID: 17331333
DOI: 10.1211/jpp.58.12.0016

Abstract

Cordycepin (3'-deoxyadenosine), which comes from Cordyceps militaris, the Chinese medicinal fungal genus Cordyceps, is known to have anti-tumour activity. In this study, we investigated the novel effect of cordycepin on human platelet aggregation that was induced by U46619, a thromboxane A(2) (TXA(2)) analogue. TXA(2) is an aggregation-inducing autacoidal molecule that is produced in various agonist-activated platelets. Cordycepin completely inhibited U46619-induced platelet aggregation and simultaneously reduced cytosolic free Ca(2+) ([Ca(2+)](i)), which was increased by U46619 (5 microM) up to 66%. Furthermore, the U46619-stimulated phosphorylation of Ca(2+)-dependent proteins (20 kDa of a myosin light chain and 47 kDa of pleckstrin) was strongly inhibited by cordycepin. These results suggest that cordycepin may have a beneficial effect on autacoidal TXA(2)-mediated thrombotic diseases by inhibiting TXA(2)-induced platelet aggregation via suppression of the Ca(2+) level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology*
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology
Blood Proteins / chemistry
Blood Proteins / metabolism
Calcium / antagonists & inhibitors
Calcium / metabolism
Calcium Chloride / pharmacology
Calcium Signaling / drug effects
Deoxyadenosines / chemistry
Deoxyadenosines / pharmacology*
Dose-Response Relationship, Drug
Humans
Medicine, Chinese Traditional
Molecular Structure
Myosin Light Chains / metabolism
Phosphoproteins / chemistry
Phosphoproteins / metabolism
Phosphorus Radioisotopes
Phosphorylation / drug effects
Platelet Aggregation / drug effects*
Platelet Aggregation Inhibitors / chemistry
Platelet Aggregation Inhibitors / pharmacology*
Thromboxane A2 / analogs & derivatives
Thromboxane A2 / pharmacology

Substances

Antineoplastic Agents
Blood Proteins
Deoxyadenosines
Myosin Light Chains
Phosphoproteins
Phosphorus Radioisotopes
Platelet Aggregation Inhibitors
platelet protein P47
Thromboxane A2
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
cordycepin
Calcium Chloride
Calcium