Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

Ilaria Iacobucci; Livia Galletti; Marilina Amabile; Simona Soverini; Michele Baccarani; Giovanni Martinelli

doi:10.3324/haematol.10632

Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

Haematologica. 2007 Mar;92(3):429-30. doi: 10.3324/haematol.10632.

Authors

Ilaria Iacobucci, Livia Galletti, Marilina Amabile, Simona Soverini, Michele Baccarani, Giovanni Martinelli

PMID: 17339200
DOI: 10.3324/haematol.10632

Abstract

In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL results.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Computer Systems
Fusion Proteins, bcr-abl / biosynthesis*
Fusion Proteins, bcr-abl / genetics
Genes, abl
Humans
Linear Models
Polymerase Chain Reaction / methods*
RNA, Messenger / biosynthesis
RNA, Messenger / blood*
RNA, Messenger / genetics
RNA, Neoplasm / biosynthesis
RNA, Neoplasm / blood*
RNA, Neoplasm / genetics
beta 2-Microglobulin / genetics

Substances

RNA, Messenger
RNA, Neoplasm
beta 2-Microglobulin
Fusion Proteins, bcr-abl