Aim: To prepare the monoclonal antibody (mAb) against human soluble mesothelin-related proteins (SMR) and identify its properties.
Methods: The B-cell epitopes of mesothelin (MSLN) were predicted by multi-parameter prediction method. Then BALB/c mice were immunized with compound MSLN polypeptide prepare the mAb by hybridoma technique. The specificity of antibody was evaluated by immuocytochemistry and Western blot respectively.
Results: Using the multi-parameter prediction of B-cell epitopes, the 471-481 epitope domain was selected and synthesized. Then the mice were immunized and mAb against human SMR was prepared and designated 2H10. The specificity of mAb 2H10 was evaluated to be high by using immuocytochemistry and Western blot.
Conclusion: The successful preparation of the mAb against SMR will provide efficient reagent for further detection of SMR by ELISA.