Abstract
AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay demonstrated 100% sensitivity and specificity when analyzed visually and by optical density.
MeSH terms
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Bacterial Proteins / analysis*
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Bacterial Proteins / genetics*
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Carbon-Oxygen Ligases / analysis*
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Carbon-Oxygen Ligases / genetics*
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DNA, Bacterial
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Microbial Sensitivity Tests
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Nucleic Acid Hybridization / methods*
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Sensitivity and Specificity
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics*
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Vancomycin Resistance*
Substances
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Bacterial Proteins
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DNA, Bacterial
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VanA ligase, Bacteria
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Carbon-Oxygen Ligases