Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

Damian C Melles; Willem B van Leeuwen; Susan V Snijders; Deborah Horst-Kreft; Justine K Peeters; Henri A Verbrugh; Alex van Belkum

doi:10.1016/j.mimet.2007.01.013

Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

J Microbiol Methods. 2007 May;69(2):371-5. doi: 10.1016/j.mimet.2007.01.013. Epub 2007 Feb 3.

Authors

Damian C Melles¹, Willem B van Leeuwen, Susan V Snijders, Deborah Horst-Kreft, Justine K Peeters, Henri A Verbrugh, Alex van Belkum

Affiliation

¹ Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. [email protected]

PMID: 17346834
DOI: 10.1016/j.mimet.2007.01.013

Abstract

We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used.

Publication types

Comparative Study

MeSH terms

Adolescent
Child
Child, Preschool
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field
Genetic Variation
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Staphylococcal Infections / microbiology*
Staphylococcus aureus / classification
Staphylococcus aureus / genetics*

Substances

DNA, Bacterial