Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

Christian U Riedel; Ian R Monk; Pat G Casey; David Morrissey; Gerald C O'Sullivan; Mark Tangney; Colin Hill; Cormac G M Gahan

doi:10.1128/AEM.02940-06

Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

Appl Environ Microbiol. 2007 May;73(9):3091-4. doi: 10.1128/AEM.02940-06. Epub 2007 Mar 9.

Authors

Christian U Riedel¹, Ian R Monk, Pat G Casey, David Morrissey, Gerald C O'Sullivan, Mark Tangney, Colin Hill, Cormac G M Gahan

Affiliation

¹ Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland.

Abstract

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

Publication types

Comparative Study

MeSH terms

Animals
Base Sequence
Food Inspection / methods*
Food Microbiology*
Genetic Vectors / genetics
Listeria monocytogenes / metabolism*
Luciferases / metabolism*
Luciferases, Bacterial
Mice
Molecular Sequence Data
Operon / genetics
Promoter Regions, Genetic / genetics*
Sequence Analysis, DNA

Substances

Luciferases
Luciferases, Bacterial