Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy

J Clin Microbiol. 2007 May;45(5):1624-7. doi: 10.1128/JCM.02316-06. Epub 2007 Mar 14.

Abstract

Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months following their return to Italy indicated that P. ovale relapses are common.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Child
  • Child, Preschool
  • Humans
  • Malaria / diagnosis*
  • Malaria / parasitology*
  • Middle Aged
  • Plasmodium ovale / genetics*
  • Plasmodium ovale / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic*
  • RNA, Protozoan / genetics
  • RNA, Ribosomal / genetics
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • RNA, Protozoan
  • RNA, Ribosomal