Molecular serotyping of Escherichia coli O111:H8

Lisa M Durso; James L Bono; James E Keen

doi:10.1016/j.mimet.2007.01.016

Molecular serotyping of Escherichia coli O111:H8

J Microbiol Methods. 2007 May;69(2):381-3. doi: 10.1016/j.mimet.2007.01.016. Epub 2007 Feb 7.

Authors

Lisa M Durso¹, James L Bono, James E Keen

Affiliation

¹ USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, State Spur 18D, Clay Center, Nebraska 68933, USA. [email protected]

PMID: 17367884
DOI: 10.1016/j.mimet.2007.01.016

Abstract

Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.

Publication types

Comparative Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Escherichia coli / classification*
Escherichia coli / genetics*
Escherichia coli Infections / microbiology*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics*
Flagellin
Humans
O Antigens / chemistry
O Antigens / genetics*
Polymerase Chain Reaction
Sensitivity and Specificity
Serotyping / methods

Substances

DNA, Bacterial
Escherichia coli Proteins
FliC protein, E coli
O Antigens
Flagellin