Abstract
A monoclonal antibody was produced against the recombinant human alpha subunit of CK-2. The antibody was of the IgG1 subclass and it was isolated from serum-free cell culture media and purified by affinity chromatography on Protein G Sepharose. The antibody can be used to detect specifically the CK-2 alpha subunit in immunoblots from tissue extracts. An ELISA detection test was also established which also allows the identification of the CK-2 alpha subunit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / isolation & purification
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Blotting, Western
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Casein Kinases
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin G / immunology
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Immunoglobulin G / isolation & purification
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Mice
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Mice, Inbred BALB C
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Precipitin Tests
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Protein Kinases / immunology*
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Protein Kinases / isolation & purification
Substances
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Antibodies, Monoclonal
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Immunoglobulin G
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Protein Kinases
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Casein Kinases