Genetic relatedness of multidrug-resistant Escherichia coli cultured from geographically diverse outpatient, midstream urine specimens

Diagn Microbiol Infect Dis. 2007 Jul;58(3):283-7. doi: 10.1016/j.diagmicrobio.2007.01.012. Epub 2007 Mar 26.

Abstract

Genomic DNA from 70 demographically matched geographically diverse pairs of urinary isolates of antimicrobial-susceptible and multidrug-resistant Escherichia coli was restricted using XbaI and analyzed by pulsed-field gel electrophoresis. Antimicrobial-susceptible isolates demonstrated limited genetic relatedness, whereas 2 epidemiologic clusters containing a total of 40 isolates (57.1%) were identified among the multidrug-resistant isolates.

MeSH terms

  • Ambulatory Care
  • Cluster Analysis
  • DNA Fingerprinting
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli / classification*
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology*
  • Female
  • Geography
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Molecular Epidemiology
  • Polymorphism, Restriction Fragment Length
  • Urinary Tract Infections / epidemiology
  • Urinary Tract Infections / microbiology*
  • Urine / microbiology

Substances

  • DNA, Bacterial