Abstract
We outline structure-function contributions from our laboratories on protein-RNA recognition events that monitor siRNA length, 5 -phosphate and 2-nucleotide 3 overhangs, as well as the architecture of Argonaute, its externally bound siRNA complex, and Argonaute-based models involving guide-strand-mediated mRNA binding, cleavage, and release.
Publication types
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Research Support, N.I.H., Extramural
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Review
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Humans
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MicroRNAs / biosynthesis
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Models, Biological
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Models, Molecular
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Peptide Initiation Factors / chemistry
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Peptide Initiation Factors / genetics
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Peptide Initiation Factors / metabolism
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Protein Structure, Tertiary
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RNA Interference*
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / chemistry
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Ribonuclease III / metabolism
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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Bacterial Proteins
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MicroRNAs
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Peptide Initiation Factors
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RNA, Messenger
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RNA, Small Interfering
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Viral Proteins
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Ribonuclease III