Crystallization and preliminary X-ray analysis of complexes of peptide inhibitors with human recombinant and mouse submandibular renins

J Mol Biol. 1992 Jan 20;223(2):447-53. doi: 10.1016/0022-2836(92)90663-5.

Abstract

Inhibitor-complexed crystals of mouse and human renins suitable for X-ray analysis have been prepared. The mouse renin is complexed with a non-hydrolysable decapeptide analogue of rat angiotensinogen containing a hydroxyethylene isostere in place of the scissile bond. The crystals are monoclinic, space group P2(1) with cell dimensions a = 78.3 A, b = 117.8 A, c = 85.9 A, beta = 101.18 degrees containing four molecules per asymmetric unit. The human renin is fully glycosylated and complexed with a tetrapeptide containing norstatine. The complex crystallises in the cubic space group P2(1)3 with a = 143.1 A and has two molecules in the asymmetric unit. The rotation function of the mouse renin complex indicates pseudo 222 symmetry while that of human renin indicates a pseudo 2-fold axis. Full structural analyses of the two complexes are underway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Angiotensinogen / analogs & derivatives*
  • Animals
  • Crystallography
  • Glycoproteins / chemistry*
  • Humans
  • Macromolecular Substances
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments / chemistry*
  • Protein Conformation
  • Recombinant Proteins / chemistry
  • Renin / antagonists & inhibitors
  • Renin / chemistry*
  • Submandibular Gland / chemistry*

Substances

  • Glycoproteins
  • Macromolecular Substances
  • Peptide Fragments
  • Recombinant Proteins
  • Angiotensinogen
  • Renin