[Recent advances in the immunodiagnosis of human cystic echinococcosis]

Enferm Infecc Microbiol Clin. 2007 Apr;25(4):263-9. doi: 10.1157/13100468.
[Article in Spanish]

Abstract

Human cystic echinococcosis is a severe zoonotic infection caused by the larval stage of the taeniid tapeworm Echinococcus granulosus. The infection may be fatal if proper treatment is not provided; hence, early diagnosis is very important. Currently, ELISA and immunoblotting are the most reliable tests for serodiagnostic purposes, although their accuracy is largely dependent on the quality of the antigenic source used. Hydatid cyst fluid has been the antigenic extract of choice for primary immunodiagnosis of the disease, which is mainly based on the detection of antigens B and 5. Several problems are associated with this extract, however, including a lack of sensitivity and specificity, and difficulties with standardization of its use. This paper reviews recent advances in the identification and characterization of novel antigens that may be useful for the immunodiagnosing of human cystic echinococcosis, with emphasis on progress in recombinant technologies and synthetic peptides. Novel approaches are discussed, such as the design of antigenic extracts from other developmental stages of the parasite, as well as the usefulness of serum cytokine detection in the clinical follow-up of affected patients after surgical or pharmacological treatment.

Publication types

  • Review

MeSH terms

  • Aftercare
  • Animals
  • Antibodies, Helminth / biosynthesis
  • Antibodies, Helminth / blood
  • Antigens, Helminth / analysis
  • Antigens, Helminth / genetics
  • Cross Reactions
  • Disease Reservoirs
  • Dog Diseases / diagnosis
  • Dog Diseases / parasitology
  • Dogs / parasitology
  • Echinococcosis / diagnosis*
  • Echinococcosis / immunology
  • Echinococcosis / parasitology
  • Echinococcus granulosus / genetics
  • Echinococcus granulosus / growth & development
  • Echinococcus granulosus / immunology
  • Echinococcus granulosus / isolation & purification
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards
  • Enzyme-Linked Immunosorbent Assay / trends
  • Humans
  • Immunoblotting / methods*
  • Immunoblotting / standards
  • Immunoblotting / trends
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / blood
  • Immunologic Tests / trends
  • Lymphokines / metabolism
  • Sensitivity and Specificity
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Zoonoses

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Immunoglobulin G
  • Lymphokines