Abstract
One proposed strategy for controlling the transmission of insect-borne pathogens uses a drive mechanism to ensure the rapid spread of transgenes conferring disease refractoriness throughout wild populations. Here, we report the creation of maternal-effect selfish genetic elements in Drosophila that drive population replacement and are resistant to recombination-mediated dissociation of drive and disease refractoriness functions. These selfish elements use microRNA-mediated silencing of a maternally expressed gene essential for embryogenesis, which is coupled with early zygotic expression of a rescuing transgene.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / physiology
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Animals
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Antigens, Differentiation / genetics*
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Antigens, Differentiation / physiology
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Crosses, Genetic
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DNA Transposable Elements
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Drosophila / embryology
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Drosophila / genetics*
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Drosophila / physiology*
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Drosophila Proteins / genetics*
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Drosophila Proteins / physiology
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Embryonic Development
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Female
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Gene Expression
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Genes, Insect*
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Genetic Engineering*
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Heterozygote
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Homozygote
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Male
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MicroRNAs / genetics
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Molecular Sequence Data
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RNA Interference*
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Receptors, Immunologic / genetics*
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Receptors, Immunologic / physiology
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Recombination, Genetic
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Repetitive Sequences, Nucleic Acid*
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Transgenes
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Zygote / physiology
Substances
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Adaptor Proteins, Signal Transducing
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Antigens, Differentiation
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DNA Transposable Elements
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Drosophila Proteins
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MicroRNAs
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Myd88 protein, Drosophila
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Receptors, Immunologic
Associated data
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GENBANK/EF447105
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GENBANK/EF447106