Anti-inflammatory effects of pravastatin on Helicobacter pylori-induced gastritis in mice

Masanori Yamato; Toshio Watanabe; Kazuhide Higuchi; Koichi Taira; Tetsuya Tanigawa; Masatsugu Shiba; Kazunari Tominaga; Yasuhiro Fujiwara; Nobuhide Oshitani; Koji Takeuchi; Tetsuo Arakawa

doi:10.1007/s10620-006-9638-7

Anti-inflammatory effects of pravastatin on Helicobacter pylori-induced gastritis in mice

Dig Dis Sci. 2007 Oct;52(10):2833-9. doi: 10.1007/s10620-006-9638-7. Epub 2007 Apr 3.

Authors

Masanori Yamato¹, Toshio Watanabe, Kazuhide Higuchi, Koichi Taira, Tetsuya Tanigawa, Masatsugu Shiba, Kazunari Tominaga, Yasuhiro Fujiwara, Nobuhide Oshitani, Koji Takeuchi, Tetsuo Arakawa

Affiliation

¹ Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.

PMID: 17404852
DOI: 10.1007/s10620-006-9638-7

Abstract

Pravastatin, an HMG-CoA reductase inhibitor, exerts anti-inflammatory effects via several mechanisms including induction of endothelial nitric oxide synthase (eNOS). We investigated the effect of pravastatin on Helicobacter pylori-induced gastritis in mice. Mice with or without H. pylori infection received intraperitoneal pravastatin daily for 1 week. Expression of eNOS mRNA and tumor necrosis factor-alpha mRNA and myeloperoxidase activity in gastric tissue was determined. Myeloperoxidase activity was reduced in a dose-dependent manner by pravastatin, with activity inhibited by 53.5 and 73.7% at doses of 0.3 and 1 mg/kg, respectively. At a dose of 1 mg/kg, pravastatin reduced the level of tumor necrosis factor-alpha mRNA by 52.7%, while it did not affect eNOS expression. Pravastatin had no effects on these inflammatory parameters in uninfected mice. Pravastatin did not affect the viability of H. pylori. In conclusion, pravastatin exerts an anti-inflammatory effect on H. pylori-induced gastritis in mice without affecting eNOS expression.

MeSH terms

Animals
Biomarkers / metabolism
Disease Models, Animal
Female
Follow-Up Studies
Gastric Mucosa / metabolism
Gastric Mucosa / microbiology
Gastric Mucosa / pathology*
Gastritis / drug therapy*
Gastritis / etiology
Gastritis / pathology
Gene Expression / drug effects
Helicobacter Infections / complications
Helicobacter Infections / drug therapy*
Helicobacter Infections / microbiology
Helicobacter pylori / isolation & purification
Helicobacter pylori / pathogenicity*
Hydroxymethylglutaryl-CoA Reductase Inhibitors / therapeutic use*
Immunohistochemistry
Interleukin-1beta / genetics
Interleukin-1beta / metabolism
Mice
Mice, Inbred C57BL
Nitric Oxide Synthase Type III / genetics
Nitric Oxide Synthase Type III / metabolism
Pravastatin / therapeutic use*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Treatment Outcome
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism

Substances

Biomarkers
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Interleukin-1beta
RNA, Messenger
Tumor Necrosis Factor-alpha
Nitric Oxide Synthase Type III
Pravastatin