Abstract
The standard sequence-based method for the typing of Legionella pneumophila serogroup 1 strains was extended by using the gspA and neuA alleles. The use of neuA as a seventh allele for typing significantly increased the index of discrimination calculated for a panel of unrelated strains (from 0.932 to 0.963) and subdivided some known large common complexes (e.g., 1,4,3,1,1,1). This modification to the standard method is proposed as the method of choice in the epidemiological investigation of L. pneumophila infections.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Bacterial Proteins / genetics
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Bacterial Typing Techniques*
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Consensus Sequence*
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Heat-Shock Proteins / genetics
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Humans
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Legionella pneumophila / classification*
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Legionella pneumophila / enzymology
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Legionella pneumophila / genetics
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Legionnaires' Disease / epidemiology
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Legionnaires' Disease / microbiology
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Molecular Sequence Data
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N-Acylneuraminate Cytidylyltransferase / genetics*
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Sequence Analysis, DNA
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Serotyping
Substances
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Bacterial Proteins
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Heat-Shock Proteins
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global stress protein A, bacteria
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N-Acylneuraminate Cytidylyltransferase
Associated data
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GENBANK/AM490070
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GENBANK/AM490071
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GENBANK/AM490072
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GENBANK/AM490073
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GENBANK/AM490074
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GENBANK/AM490075
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GENBANK/AM490076
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GENBANK/AM490077
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GENBANK/AM490078
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GENBANK/AM490079
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GENBANK/AM490080
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GENBANK/AM490081
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GENBANK/AM490082
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GENBANK/AM490083
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GENBANK/AM490084
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GENBANK/AM490085