Abstract
In this study, we characterize proviral DNA of 20 HIV-1 asymptomatic antiretroviral-naive patients from Venezuela in env, gag, and pol genes regions. Results from both env/gag HMA subtyping and phylogenetic analysis of pol partial sequences led to the description of clade B in all cases. Nevertheless, the high prevalence of polymorphisms was particularly evident among the protease sequences. A 10% prevalence of major resistance mutations to RTIs was found. Our data also suggested that the protease polymorphisms I62T and V77T could be considered as molecular markers of the subtype B local epidemic. In addition, we show how proviral DNA can be used as a reliable tool to follow trends of resistance mutation transmission.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Mutational Analysis
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Drug Resistance, Multiple, Viral / genetics*
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Female
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HIV Infections / classification
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HIV Infections / drug therapy*
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HIV Infections / epidemiology
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HIV Protease / classification
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HIV Protease / genetics
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HIV Reverse Transcriptase / classification
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HIV Reverse Transcriptase / genetics
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HIV-1 / classification
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HIV-1 / drug effects*
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Humans
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Male
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Molecular Sequence Data
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Phylogeny
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Venezuela / epidemiology
Substances
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HIV Reverse Transcriptase
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HIV Protease
Associated data
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GENBANK/AY587958
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GENBANK/AY587959
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GENBANK/AY587960
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