Abstract
beta-Lactam resistance in Haemophilus parasuis is an emerging phenomenon that has not yet been characterized from a molecular perspective. Clinical high-level beta-lactam-resistant isolates from Spain bore a novel plasmid, pB1000, expressing a functionally active ROB-1 beta-lactamase. Pulsed-field gel electrophoresis was applied for the first time to H. parasuis and showed that beta-lactam resistance is due to clonal spread of a resistant strain, BB1018, bearing pB1000.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Electrophoresis, Gel, Pulsed-Field
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Haemophilus Infections / microbiology
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Haemophilus Infections / veterinary
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Haemophilus parasuis / drug effects*
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Haemophilus parasuis / enzymology
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Haemophilus parasuis / genetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids*
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Sequence Analysis, DNA
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Swine / microbiology
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Swine Diseases / microbiology
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
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beta-Lactams / pharmacology*
Substances
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beta-Lactams
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beta-lactamse ROB-1
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beta-Lactamases
Associated data
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GENBANK/DQ840517
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GENBANK/DQ845801
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GENBANK/DQ845802
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GENBANK/DQ845803
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GENBANK/DQ845805
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GENBANK/DQ845806
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GENBANK/DQ845807
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GENBANK/DQ845808