Abstract
The genetic heterogeneity of hepatitis B virus (HBV) genotypes and subgenotypes was investigated by directly sequencing amplified PreS, S and P genes of HBV isolates obtained from the plasma of 99 subjects with chronic HBV infection. Genotype D showed the greatest intragenotypic and intrasubgenotypic divergence: in particular, the a determinant was mutated in 58.2% of the genotype D patients, two of whom showed prototypic vaccine-induced escape mutants at codon 145. Moreover, five sites under significant positive selection were found in the S protein of the D isolates: one in the a determinant and four in the highly hydrophobic C terminal. Our results suggest that careful surveillance of vaccine-induced escape mutants should be considered in populations with highly frequent genotype D infections, and raise questions concerning the possible relationship between the genetic heterogeneity, host immunity and pathogenicity of this HBV genotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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DNA, Viral / analysis
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Female
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Genetic Heterogeneity*
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Genotype
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Hepatitis B Surface Antigens / analysis*
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Hepatitis B Surface Antigens / chemistry
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Hepatitis B Surface Antigens / genetics*
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Hepatitis B Virus, Duck / classification
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Hepatitis B Virus, Duck / genetics*
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Humans
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Male
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Molecular Sequence Data
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Phylogeny
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Sequence Analysis, DNA
Substances
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DNA, Viral
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Hepatitis B Surface Antigens
Associated data
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GENBANK/AB033558
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GENBANK/AB033559
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GENBANK/AB036907
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GENBANK/AB036910
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GENBANK/AB036912
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GENBANK/AB036920
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