[Preparation and identification of a single-chain antibody fragment against high pathogenic H5N1 avian influenza virus]

Zhong Fang; Wen-Xin Luo; Ming-Qiao Wang; Ying-Wei Chen; Jun Zhang; Hong-Lin Chen; Yi Guan; Ning-Shao Xia

doi:10.1016/s1872-2075(07)60026-0

[Preparation and identification of a single-chain antibody fragment against high pathogenic H5N1 avian influenza virus]

Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):292-6. doi: 10.1016/s1872-2075(07)60026-0.

[Article in Chinese]

Authors

Zhong Fang¹, Wen-Xin Luo, Ming-Qiao Wang, Ying-Wei Chen, Jun Zhang, Hong-Lin Chen, Yi Guan, Ning-Shao Xia

Affiliation

¹ National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Key Laboratory of Education Minister for Cell Biology and Tumor Cell Engineering of Xiamen University, School of Life Sciences, Xiamen University, Xiamen 361005, China.

PMID: 17460904
DOI: 10.1016/s1872-2075(07)60026-0

Abstract

Previously, an mAb 10F7 was developed against H5N1 hemagglutinin, which was highly specific to 34 different H5N1 strains and showed good neutralizing activity. In the present study, the single-chain fragment of the antibody was cloned into a prokaryotic vector and then expressed in E. coli. The activity of the scFv was tested in hemagglution inhibition and neutralization experiment. Two H5N1 virus strains were inhibited to bind erythrocyte cells by the scFv while the H9 virus was not. Also, five H5N1 virus strains were neutralized during infecting MDCK cells. These results showed an approachable method for developing therapeutic antibody to H5N1 virus.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Viral / genetics
Antibodies, Viral / immunology*
Antibodies, Viral / metabolism
Antibody Specificity / immunology
Birds / virology
Cell Line
Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel
Influenza A Virus, H5N1 Subtype / immunology*
Influenza in Birds / virology
Neutralization Tests
Recombinant Proteins / immunology
Recombinant Proteins / metabolism
Single-Chain Antibodies / genetics
Single-Chain Antibodies / immunology*
Single-Chain Antibodies / metabolism

Substances

Antibodies, Viral
Recombinant Proteins
Single-Chain Antibodies