Abstract
Aiming to replace the radioisotopic assay, the widely used procedure for vitro antimalarial drug screening, we set up a protocol using a Plasmodium falciparum strain transformed with the green fluorescent protein (PfGFP), which can be quickly and specifically quantified by flow cytometry. On the basis of a side-by-side comparison, this PfGFP-based method showed results similar to those obtained with the standard radioisotopic method.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antimalarials / pharmacology*
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Drug Evaluation, Preclinical
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Erythrocytes / parasitology
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Flow Cytometry / methods*
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Green Fluorescent Proteins / genetics*
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Green Fluorescent Proteins / metabolism
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Humans
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Hypoxanthine / metabolism
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Parasitic Sensitivity Tests / methods
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Plasmodium falciparum / drug effects*
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Plasmodium falciparum / genetics
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Plasmodium falciparum / growth & development
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Plasmodium falciparum / metabolism
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Transfection
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Transformation, Genetic*
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Tritium / metabolism
Substances
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Antimalarials
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Tritium
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Green Fluorescent Proteins
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Hypoxanthine