Abstract
A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.
MeSH terms
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Acetamides / pharmacology*
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Anti-Infective Agents / pharmacology*
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Drug Resistance, Bacterial / genetics*
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Enterococcus / drug effects*
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Enterococcus / genetics
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In Situ Hybridization, Fluorescence / methods*
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Linezolid
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Microbial Sensitivity Tests
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Mutation*
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Oxazolidinones / pharmacology*
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RNA, Ribosomal, 23S / genetics
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Sensitivity and Specificity
Substances
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Acetamides
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Anti-Infective Agents
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Oxazolidinones
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RNA, Ribosomal, 23S
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Linezolid