Design, synthesis, and anti-HIV activity of 2',3'-didehydro-2',3'-dideoxyuridine (d4U), 2',3'-dideoxyuridine (ddU) phosphoramidate 'ProTide' derivatives

Bioorg Med Chem Lett. 2007 Jul 1;17(13):3666-9. doi: 10.1016/j.bmcl.2007.04.043. Epub 2007 Apr 22.

Abstract

We report the synthesis of 2',3'-didehydro-2',3'-dideoxyuridine (d4U) and 2',3'-dideoxyuridine (ddU) phosphoramidate 'ProTide' derivatives and their evaluation against HIV-1 and HIV-2. In addition, we conducted molecular modeling studies on both d4U and ddU monophosphates to investigate their second phosphorylation process. The findings from the modeling studies provide compelling evidence for the lack of anti-HIV activity of d4U phosphoramidates, in contrast with the corresponding ddU phosphoramidates.

MeSH terms

  • Amides / chemistry*
  • Anti-HIV Agents / chemical synthesis*
  • Anti-HIV Agents / chemistry
  • Chemistry, Pharmaceutical / methods*
  • Dideoxynucleosides / chemical synthesis*
  • Dideoxynucleosides / chemistry*
  • Drug Design
  • HIV-1 / metabolism
  • HIV-2 / metabolism
  • Models, Chemical
  • Models, Molecular
  • Nucleoside-Phosphate Kinase / chemistry
  • Nucleosides / chemistry
  • Phosphates / chemistry
  • Phosphoric Acids / chemistry*
  • Phosphorylation
  • Thymidine Monophosphate / chemistry

Substances

  • Amides
  • Anti-HIV Agents
  • Dideoxynucleosides
  • Nucleosides
  • Phosphates
  • Phosphoric Acids
  • Thymidine Monophosphate
  • 2',3'-didehydro-2',3'-dideoxyuridine
  • 2',3'-dideoxyuridine
  • phosphoramidic acid
  • Nucleoside-Phosphate Kinase
  • dTMP kinase