Abstract
Schnurri (Shn)-2 is a large zinc finger-containing protein, which plays a critical role in cell growth, signal transduction and lymphocyte development. In Shn-2-deficient (Shn-2(-/-)) CD4 T cells, the activation of nuclear factor-kappaB is up-regulated and their ability to differentiate into Th2 is enhanced. Here, we extend our investigation and demonstrate that Shn-2 regulates Th2 responses in vivo using an ovalbumin-induced allergic asthma model. Eosinophilic inflammation, mucus hyperproduction and airway hyperresponsiveness (AHR) were all enhanced in Shn-2(-/-) mice. Moreover, eosinophilic infiltration and AHR were enhanced in mice given a transfer of Shn-2(-/-) effector Th2. Shn-2 in Th2 is thus considered to play an important role as a negative regulator in allergic airway inflammation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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Asthma / immunology
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Asthma / pathology
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Asthma / physiopathology
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Bronchoalveolar Lavage Fluid / chemistry
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Bronchoalveolar Lavage Fluid / cytology
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / pathology
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Cell Count
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Chemokine CCL17
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Chemokine CCL22
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Chemokines, CC / genetics
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Chemokines, CC / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / physiology
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Eosinophils / metabolism
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Eosinophils / pathology
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Flow Cytometry
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Gene Expression
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Interleukin-13 / metabolism
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Interleukin-5 / metabolism
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Lung / metabolism
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Lung / pathology
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Lung / physiopathology
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Mice, Transgenic
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Ovalbumin / immunology
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Pneumonia / immunology*
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Pneumonia / pathology
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Respiratory Hypersensitivity / immunology*
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Respiratory Hypersensitivity / pathology
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Respiratory Hypersensitivity / physiopathology
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Th2 Cells / immunology*
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Th2 Cells / transplantation
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Vaccination
Substances
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Ccl17 protein, mouse
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Ccl22 protein, mouse
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Chemokine CCL17
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Chemokine CCL22
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Chemokines, CC
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DNA-Binding Proteins
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Hivep2 protein, mouse
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Interleukin-13
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Interleukin-5
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Receptors, Antigen, T-Cell, alpha-beta
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Ovalbumin