Diagnosis of a critical respiratory illness caused by human metapneumovirus by use of a pan-virus microarray

Charles Y Chiu; Ash A Alizadeh; Silvi Rouskin; Jason D Merker; Elaine Yeh; Shigeo Yagi; David Schnurr; Bruce K Patterson; Don Ganem; Joseph L DeRisi

doi:10.1128/JCM.00364-07

Diagnosis of a critical respiratory illness caused by human metapneumovirus by use of a pan-virus microarray

J Clin Microbiol. 2007 Jul;45(7):2340-3. doi: 10.1128/JCM.00364-07. Epub 2007 May 9.

Authors

Charles Y Chiu¹, Ash A Alizadeh, Silvi Rouskin, Jason D Merker, Elaine Yeh, Shigeo Yagi, David Schnurr, Bruce K Patterson, Don Ganem, Joseph L DeRisi

Affiliation

¹ Department of Biochemistry and Biophysics, University of California, San Francisco at Mission Bay, office BH403C, QB3 building, 1700 4th Street, San Francisco CA 94158, USA.

Abstract

A pan-virus DNA microarray (Virochip) was used to detect a human metapneumovirus (hMPV) strain associated with a critical respiratory tract infection in an elderly adult with chronic lymphocytic leukemia. This infection had previously eluded diagnosis despite extensive microbiological testing for possible etiologic agents. The patient's hMPV strain did not grow in viral culture, and only one of five specific reverse transcription-PCR assays for hMPV was positive.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Anti-Infective Agents / therapeutic use
Humans
Male
Metapneumovirus / genetics
Metapneumovirus / isolation & purification*
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis / methods*
Paramyxoviridae Infections / diagnosis
Paramyxoviridae Infections / virology*
Phylogeny
Respiratory Tract Infections / drug therapy
Respiratory Tract Infections / virology*

Substances

Anti-Infective Agents

Associated data

GENBANK/EF088284
GENBANK/EF088285