Enhancement of IgE synthesis in the human myeloma cell line U-266 with an IgE binding factor from a human T-cell line

Scand J Immunol. 1991 Dec;34(6):721-6. doi: 10.1111/j.1365-3083.1991.tb01596.x.

Abstract

An IgE-binding factor(s) (IgE-BF(s] was partially purified from the supernatant of human HTLV-II carrying T-cell line MO. This IgE-BF(s) was shown to increase the IgE synthesis in the human myeloma cell line U-266, but did not affect its viability or growth. The effect of the IgE-BF(s) was dose-dependent and selective for IgE protein synthesis as beta 2-microglobulin synthesis in the U-266 and the immunoglobulin production in the U-1958 IgG-secreting human myeloma cell line were unaffected. The IgE-BF(s) increased the production of the epsilon heavy chain but not the lambda light chain production. The IgE-BF(s) was distinct from IL-1 beta, IL-3, IL-4, IL-5, IL-6, TNF-alpha, IFN-alpha, -beta, -gamma, M-CSF, and fragments of CD23.

MeSH terms

  • Cytokines / biosynthesis
  • Humans
  • Immunoglobulin E / biosynthesis*
  • Interleukin-6 / analysis
  • Lymphokines / pharmacology*
  • Multiple Myeloma / immunology*
  • Prostatic Secretory Proteins*
  • RNA, Messenger / analysis
  • T-Lymphocytes / physiology*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / analysis

Substances

  • Cytokines
  • Interleukin-6
  • Lymphokines
  • Prostatic Secretory Proteins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • beta-microseminoprotein
  • immunoglobulin-binding factors
  • Immunoglobulin E