Feline chronic gingivo-stomatitis (FCGS) is a syndrome characterised by persistent, often severe, inflammation of the oral mucosa. In the absence of similar studies, our objective was to estimate the prevalence of FCGS in a convenience based sample of cats visiting first opinion small animal veterinary practices. Twelve practices took part, providing a sample population of 4858 cats. Veterinary surgeons identified cases of FCGS according to our case definition over a 12-week sampling period; age, sex and breed information was determined for all cats, plus brief descriptive data for FCGS cases. The prevalence of FCGS was 0.7% (34 cases, 95% confidence intervals: 0.5-1.0%). Of the 34 cases of FCGS, 44% (15 cats) were new cases and 56% (19 cats) were ongoing cases. No statistically significant difference (P>0.353) was found when the age, sex and breed of cats with FCGS were compared to data from cats without the condition.