Recombinant Mycobacterium bovis bacillus Calmette-Guerin elicits human immunodeficiency virus type 1 envelope-specific T lymphocytes at mucosal sites

Clin Vaccine Immunol. 2007 Jul;14(7):886-93. doi: 10.1128/CVI.00407-06. Epub 2007 May 16.

Abstract

A successful vaccine vector for human immunodeficiency virus type 1 (HIV-1) should induce anti-HIV-1 T-cell immune responses at mucosal sites. We have constructed recombinant Mycobacterium bovis bacillus Calmette-Guérin (rBCG) expressing an HIV-1 group M consensus envelope (Env) either as a surface, intracellular, or secreted protein as an immunogen. rBCG containing HIV-1 env plasmids engineered for secretion induced optimal Env-specific T-cell gamma interferon enzyme-linked immunospot responses in murine spleen, female reproductive tract, and lungs. While rBCG-induced T-cell responses to HIV-1 envelope in spleen were lower than those induced by adenovirus prime/recombinant vaccinia virus (rAd-rVV) boost, rBCG induced comparable responses to rAd-rVV immunization in the female reproductive tract and lungs. T-cell responses induced by rBCG were primarily CD4(+), although rBCG alone did not induce anti-HIV-1 antibody. However, rBCG could prime for a protein boost by HIV-1 envelope protein. Thus, rBCG can serve as a vector for induction of anti-HIV-1 consensus Env cellular responses at mucosal sites.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / immunology
  • BCG Vaccine / immunology*
  • BCG Vaccine / pharmacology
  • Female
  • Gene Products, env / immunology
  • Gene Products, env / pharmacology
  • Genitalia, Female / immunology
  • HIV Envelope Protein gp120 / immunology
  • HIV Envelope Protein gp120 / pharmacology
  • HIV Infections / immunology*
  • HIV Infections / prevention & control*
  • HIV-1 / immunology*
  • Immunization, Secondary
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Lung / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mucous Membrane / immunology
  • Mycobacterium bovis / immunology
  • Spleen / immunology
  • Spleen / metabolism
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / virology
  • Vaccines, Synthetic / immunology
  • Vaccines, Synthetic / pharmacology
  • env Gene Products, Human Immunodeficiency Virus

Substances

  • Antibodies, Viral
  • BCG Vaccine
  • Gene Products, env
  • HIV Envelope Protein gp120
  • Vaccines, Synthetic
  • env Gene Products, Human Immunodeficiency Virus
  • gp140 envelope protein, Human immunodeficiency virus 1
  • Interferon-gamma