[Effect of nordy on FPR function of malignant human glioma cell line U87]

Yao Xue Xue Bao. 2007 Mar;42(3):257-62.
[Article in Chinese]

Abstract

Nordy is a synthesized chrial compound. To investigate the effects of nordy (25 - 100 micromol x L(-1)) on the function of formylpeptide receptor (FPR) of malignant human glioma cells, human glioblastoma cell line U87 was used to detect its proliferation, migration, calcium mobilization, vascular endothelial growth factor (VEGF) mRNA and protein levels after activation of FPR by its agonist N-formyl-methionyl-leucyl-phenylalanine (fMLF). Cell proliferation, migration ability, VEGF mRNA, VEGF protein and calcium mobilization were evaluated by cell counting, chemotaxis assay, RT-PCR, ELISA and spectrometry. Nordy (50 - 100 micromol x L(-1)) potently inhibited the proliferation, migration and calcium mobilization of U87 cells induced by fMLF (P < 0.05). Moreover, 100 micromol x L(-1) nordy showed a significantly impaired VEGF mRNA expression and protein secretion induced by fMLF (P < 0.05). Nordy could inhibit FPR functioning in glioma cell proliferation, migration and angiogenesis, which might be a possible mechanism of its anti-cancer effects.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Calcium / metabolism
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects*
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Glioblastoma / genetics
  • Glioblastoma / metabolism
  • Glioblastoma / pathology
  • Humans
  • Masoprocol / analogs & derivatives*
  • Masoprocol / pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, Formyl Peptide / agonists
  • Receptors, Formyl Peptide / metabolism
  • Receptors, Formyl Peptide / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spectrophotometry / methods
  • Vascular Endothelial Growth Factor A / biosynthesis
  • Vascular Endothelial Growth Factor A / genetics

Substances

  • Antineoplastic Agents
  • RNA, Messenger
  • Receptors, Formyl Peptide
  • Vascular Endothelial Growth Factor A
  • nordy
  • N-Formylmethionine Leucyl-Phenylalanine
  • Masoprocol
  • Calcium