Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium

Int J Syst Evol Microbiol. 2007 Jun;57(Pt 6):1222-1226. doi: 10.1099/ijs.0.64973-0.

Abstract

A moderately halophilic bacterium, designated strain SL014B-85(T), was isolated from a crude-oil-contaminated saline soil from Shengli oilfield, Shandong Province, China. Cells were Gram-negative, aerobic, short rods with lateral flagella. Growth occurred at NaCl concentrations of 0-15 % (optimum 5-15 %), at 10-42 degrees C (optimum 30 degrees C) and at pH 8.0-9.0 (optimum pH 8.5). The only respiratory quinone was Q9, and the main cellular fatty acids were C(18 : 1)omega7c, C(16 : 0) and C(19 : 0) cyclo omega8c. The G+C content of the DNA was 66.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B-85(T) belonged to the genus Halomonas in the Gammaproteobacteria, with highest sequence similarity of 98.1 and 97.8 % to Halomonas alimentaria DSM 15356(T) and Halomonas ventosae DSM 15911(T), respectively. DNA-DNA relatedness values were below 40 % with members of closely related Halomonas species. Results of phenotypic, biochemical and phylogenetic analyses revealed that strain SL014B-85(T) could be classified as representing a novel species of the genus Halomonas, for which the name Halomonas shengliensis sp. nov. is proposed. The type strain is SL014B-85(T) (=CGMCC 1.6444(T)=LMG 23897(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Flagella
  • Genes, rRNA
  • Halomonas / classification*
  • Halomonas / genetics
  • Halomonas / isolation & purification
  • Halomonas / metabolism*
  • Hydrogen-Ion Concentration
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Nitrogen / metabolism*
  • Nucleic Acid Hybridization
  • Petroleum / metabolism*
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride / metabolism
  • Soil Microbiology*
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Petroleum
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Nitrogen

Associated data

  • GENBANK/EF121853