Abstract
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA, Viral / classification*
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DNA, Viral / genetics
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Humans
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Influenza A Virus, H5N1 Subtype / classification*
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza in Birds / diagnosis*
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Influenza in Birds / genetics
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Influenza in Birds / virology
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Influenza, Human / diagnosis*
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Influenza, Human / genetics
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Influenza, Human / virology
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Molecular Diagnostic Techniques / methods*
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Molecular Sequence Data
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Poultry / virology*
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Virology / methods*