Abstract
Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G beta2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Cell Nucleus / chemistry
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Chromatography, Liquid
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Computational Biology
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Cytosol / chemistry
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Neoplasm Proteins / analysis*
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Neuroblastoma / chemistry
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Neuroblastoma / metabolism*
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Peripheral Nervous System Neoplasms / chemistry
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Peripheral Nervous System Neoplasms / metabolism*
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Protein Array Analysis*
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Proteomics*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tretinoin / pharmacology
Substances
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Neoplasm Proteins
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Tretinoin