Determination of ochratoxin A in dry-cured meat products by a HPLC-FLD quantitative method

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug 15;855(2):242-8. doi: 10.1016/j.jchromb.2007.05.010. Epub 2007 May 18.

Abstract

A fast and sensitive method for the quantification of the mycotoxin ochratoxin A (OTA) in dry-cured meat products has been developed, which does not require a clean-up step, by HPLC with an alkaline mobile phase (pH 9.8). Validation procedures for specificity, trueness, ruggedness, stability, recovery and repeatability were performed. The decision limit (CC alpha) and the decision capability (CC beta) were calculated at 1.10 and 1.23 microg/kg, respectively. The procedure was applied to representative dehydration levels of dry-cured meat samples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Meat Products / microbiology*
  • Mycotoxins / analysis*
  • Mycotoxins / isolation & purification
  • Ochratoxins / analysis*
  • Ochratoxins / isolation & purification
  • Reproducibility of Results

Substances

  • Mycotoxins
  • Ochratoxins
  • ochratoxin A