Phagocytic function of human blood polymorphonuclear leukocytes in the presence of carrageenan, a potential vaginal microbicide

Inflammation. 2007 Oct;30(5):131-5. doi: 10.1007/s10753-007-9029-z. Epub 2007 Jun 12.

Abstract

Carrageenan is currently undergoing clinical trials as the active constituent of a vaginal gel product for use as a female-controlled option to prevent the transmission of HIV during sexual intercourse. Here we show that in the presence of 0.5 mg/ml of carrageenan, human blood polymorphonuclear leukocytes (PMN) do not ingest this material, as evidenced by a lack of progressive vacuolization, but can ingest microorganisms present in the medium, excluding adjacent carrageenan. Moreover, PMN move at normal speeds, respond chemotactically, and reduce nitroblue tetrazolium (NBT) to formazan on stimulation. Hence, in the presence of carrageenan the phagocytic response appears to remain intact.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents, Local / pharmacology*
  • Carrageenan / pharmacology*
  • Cells, Cultured
  • Chemotaxis, Leukocyte / drug effects
  • Humans
  • Indicators and Reagents
  • Neutrophil Activation / drug effects*
  • Neutrophils / drug effects*
  • Nitroblue Tetrazolium
  • Phagocytosis / drug effects*
  • Vaginal Creams, Foams, and Jellies

Substances

  • Anti-Infective Agents, Local
  • Indicators and Reagents
  • Vaginal Creams, Foams, and Jellies
  • Nitroblue Tetrazolium
  • Carrageenan