[The TP53 and RPS6 alterations at the invasive tumor front, center and stroma of oral squamous cell carcinoma]

Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Mar;42(3):140-3.
[Article in Chinese]

Abstract

Objective: To assess the difference of genetic alteration patterns among different areas in the same oral squamous cell carcinoma (OSCC).

Methods: Studied the loss of heterozygosity (LOH) and microsatellite instability (MI) at chromosomal loci TP53 and RPS6 on the invasive tumor front (ITF), the center/superficial part and stroma cells by combining laser capture microdissection (LCM) and PCR technique.

Results: There existed a high frequency of LOH and MI on chromosomes loci TP53 and RPS6. The frequency of RPS6 and TP53 aberration at the stroma was 23.5% (4/17) and 43.8% (7/16), respectively. While in epithelial part (both ITF and center), it reached up to 64.7% (11/17) and 70.6% (12/17) respectively, and the difference was significant (P < 0.05). The overall frequency of the two markers was statistically higher at the ITF (20/32) than at the center/superficial part (15/34) (P < 0.05).

Conclusions: The current study revealed that genetic alterations were different in different areas of the same tumor and there existed a relationship between the histological grading and genotypes of OSCC.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Cell Proliferation
  • Genes, p53 / genetics*
  • Humans
  • Loss of Heterozygosity*
  • Microdissection
  • Microsatellite Instability
  • Middle Aged
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / pathology
  • Neoplasm Invasiveness
  • Ribosomal Protein S6 / genetics*

Substances

  • Ribosomal Protein S6